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Image Search Results


KEY RESOURCES TABLE

Journal: Cell systems

Article Title: Loss of TET2 affects proliferation and drug sensitivity through altered dynamics of cell-state transitions

doi: 10.1016/j.cels.2020.06.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE Mouse anti-Human CD34 BD Biosciences 555822 PE-Cy5 Mouse anti-Human CD38 BD Biosciences 555461 Chemicals, Peptides, and Recombinant Proteins Cytosine Beta-D-Arabinofuranoside Sigma-Aldrich C6645 Disulfiram Sigma-Aldrich D2950000 Human IFN-g PeproTech 300-02 Critical Commercial Assays CellTiter-Glo 2.0 Cell Viability Assay Promega G9242 QuantSeq 3’ mRNA-Seq Library Prep Kit FWD for Illumina Lexogen 0.15.24/96 MethoCult H4034 Optimum STEMCELL #04034 Deposited Data Raw and analyzed data This paper; Mendeley data doi: 10.17632/xmvz47rpg6.1 Epigenome analysis of leukemia stem, blast and normal hematopoietic stem/progenitor cells Jung et al., 2015 ; GEO {"type":"entrez-geo","attrs":{"text":"GSE63409","term_id":"63409"}} GSE63409 Experimental Models: Cell Lines Human male: KG-1 ATCC CCL-246; RRID: CVCL_0374 Human male: THP-1 ATCC TIB-202; RRID: CVCL_0006 Oligonucleotides Primer HOXA5-F: TCTCGTTGCCCTAATTCATCTTTT McLaughlin-Drubin et al., 2011 N/A Primer HOXA5-R: CATTCAGGACAAAGAGATGAACAGA McLaughlin-Drubin et al., 2011 N/A Primer CD38-F: CACCAAGCGCTTTCCCGAGACC This paper N/A Primer CD38-R: GAGAGGCCCCTCCAGTGCAGAA This paper N/A Primer CORO1A-F: GTGGTCCGCTCCAGCAAGTTCC This paper N/A Primer CORO1A-R: CAGACCGTGGGCGCATTCTTGT This paper N/A Primer ITGAM-F: CTCCGTGGACGTGGACAGCAAC This paper N/A Primer ITGAM-R: AATGGCCACGTCCGTCAGCTTG This paper N/A Primer TAL1-F: GGGAGCCGGATGCCTTCCCTAT This paper N/A Primer TAL1-R: ACTTCATGGCCAGGCGGAGGAT This paper N/A Recombinant DNA pSpCas9(BB)-2A-Puro (PX459) V2.0 Addgene #62988 Software and Algorithms Code to generate all figures This paper; Mendeley data doi: 10.17632/xmvz47rpg6.1 Code pertaining to model This paper; GitHub https://github.com/AltschulerWu-Lab/tet2-dynamics DNA methylation age calculator Horvath, 2013 https://horvath.genetics.ucla.edu/html/dnamage/ R R Core Team, 2019 https://www.R-project.org MATLAB R2019a https://www.mathworks.com Open in a separate window KEY RESOURCES TABLE TET2 knockout in human AML cell lines increases stem-like signatures TET2 KO cells have altered dynamics between stem-like/differentiated states Altered cell-state switching dynamics provides fitness advantage in and out of drug

Techniques: Recombinant, Viability Assay, Software, DNA Methylation Assay